Catalog # CX3435

α1-Catenin (a.a. 143-153) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

α-catenins are cadherin interacting proteins with homology to vinculin. Three α-catenin genes have been described including α1-catenin (αE-Catenin), α2-catenin (αN-catenin), and α3-catenin (αT-catenin). α1-catenin has 81% homology with α2-catenin and 60% homology with α3-catenin. These α-catenin isoforms may have similar roles since each binds cadherins. However, their expression patterns are both overlapping and distinct. α1-catenin was identified in epithelial cells, and is expressed in various cell types. α2-catenin is enriched in the nervous system, and α3-catenin is expressed highest in testis and heart. Phosphorylation may regulate the activity of α1-catenin, since tyrosine phosphorylation of Tyr-148 occurs during intercellular adhesion. This site is dephosphorylated by SHP2, which inhibits α1-catenin binding to β-catenin and translocation to the plasma membrane. Phosphorylation of α1-catenin at Tyr-148 may be important for inhibition of cell transformation, and dephosphorylation of this site may be important during SHP2-mediated cell transformation.

References

Herrenknecht, K. et al. (1991) Proc Natl Acad Sci U S A. 88(20):9156.
Hirano, S. et al. (1992) Cell. 70(2):293.
Janssens, B. et al. (2001) J Cell Sci. 114(17):3177.
Burks, J. & Agazie, Y.M. (2006) Oncogene 25:7166.

α1-Catenin synthetic peptide corresponds to amino acid residues 143 to 153 in human α1-Catenin. This peptide sequence is highly conserved in rat and mouse α1-Catenin, and has some homology to α2-Catenin or α3-Catenin.

*For more information, see UniProt Accession P35221

Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by α1-Catenin (a.a. 143-153) (CP3431) in ELISA, and has been shown to block the reactivity of CP3431 in Western blot. In addition, the peptide is recommended for use in blocking CP3431 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: